Nanotechnology for Nucleic Acid Delivery

Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Nanotechnology for Nucleic Acid Delivery

Author: Manfred Ogris

Publisher: Humana Press

ISBN: 9781627031394

Page: 286

View: 421

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Nanotechnology for the Delivery of Therapeutic Nucleic Acids

Nucleic acid (NA) therapeutics has been extensively studied both in the academia and in the pharmaceutical industry and is still considered the promise for new therapeutic modalities, especially in personalized medicine.

Nanotechnology for the Delivery of Therapeutic Nucleic Acids

Author: Dan Peer

Publisher: CRC Press

ISBN: 9814411051

Page: 240

View: 638

Nucleic acid (NA) therapeutics has been extensively studied both in the academia and in the pharmaceutical industry and is still considered the promise for new therapeutic modalities, especially in personalized medicine. The only hurdle that limits the translation of NA therapeutics from an academic idea to the new therapeutic modality is the lack of efficient and safe delivery strategies. Nanotechnology for the Delivery of Therapeutic Nucleic Acids, written by world experts in the field of nanotechnology for NA delivery, the contributing authors bring together the state of the art in delivery strategies with strong emphasis on aspects that are of essence to the pharmaceutical industry, such as stability, general toxicity, immune-toxicity, pharmacokinetics, efficacy, and validation of new drug targets using unique approaches based on exquisite nanotechnology strategies.

Nanotechnology in Drug Delivery

The reader will be introduced to various aspects of the fundamentals of nanotechnology based drug delivery systems and the application of these systems for the delivery of small molecules, proteins, peptides, oligonucleotides and genes.

Nanotechnology in Drug Delivery

Author: Melgardt M. de Villiers

Publisher: Springer Science & Business Media

ISBN: 9780387776682

Page: 662

View: 423

The reader will be introduced to various aspects of the fundamentals of nanotechnology based drug delivery systems and the application of these systems for the delivery of small molecules, proteins, peptides, oligonucleotides and genes. How these systems overcome challenges offered by biological barriers to drug absorption and drug targeting will also be described.

Biomedical Applications of Nanotechnology

With chapters contributed by distinguished scientists from diverse disciplines, Biomedical Applications of Nanotechnology : Reviews recent advances in the designing of various nanotechnologies based on nucleic acids, polymers, biomaterials, ...

Biomedical Applications of Nanotechnology

Author: Vinod Labhasetwar

Publisher: John Wiley & Sons

ISBN: 9780470152911

Page: 272

View: 373

An overview of nanotechnology and its potential The field of nanotechnology is undergoing rapid developments on many fronts. This reference provides a comprehensive review of various nanotechnologies with a view to their biomedical applications. With chapters contributed by distinguished scientists from diverse disciplines, Biomedical Applications of Nanotechnology : Reviews recent advances in the designing of various nanotechnologies based on nucleic acids, polymers, biomaterials, and metals Discusses biomedical nanotechnology in areas such as drug and gene delivery Covers advanced aspects of imaging and diagnostics Includes a chapter on the issue of nanotoxicology Complete with figures and tables, this is a practical, hands-on reference book for researchers in pharmaceutical and biotech industries, biomedical engineers, pharmaceutical scientists, pharmacologists, and materials scientists as well as for the policymakers who need to understand the potential of nanotechnology. It is also an excellent resource book for graduate-level students in pharmaceutical sciences, biomedical engineering, and other fields in which nanotechnology is playing an increasingly important role.

DNA Nanotechnology

DNA nanotechnology: From structure to function presents an overview of various facets of DNA nanotechnology, with a particular focus on their promising applications. This book is composed of three parts.

DNA Nanotechnology

Author: Chunhai Fan

Publisher: Springer Science & Business Media

ISBN: 3642360777

Page: 362

View: 136

DNA nanotechnology: From structure to function presents an overview of various facets of DNA nanotechnology, with a particular focus on their promising applications. This book is composed of three parts. Part I, Elements of DNA Nanotechnology, provides extensive basic information on DNA nanotechnology. Part II, Static and Dynamic DNA Nanotechnology, describes the design and fabrication of static and dynamic DNA nanostructures. Recent advances in DNA origami, DNA walkers and DNA nanodevices are all covered in this part. Part III, Applications of DNA Nanotechnology, introduces a variety of applications of DNA nanotechnology, including biosensing, computation, drug delivery, etc. Together these provide a comprehensive overview of this emerging area and its broad impact on biological and medical sciences. This book is intended for post-graduates, post-doctoral researchers and research scientists who are interested in expanding their knowledge of DNA nanotechnology. It provides readers an impression of the latest developments in this exciting filed.

RNA Nanoparticle as a Safe and Effective Drug Delivery Platform for Cancer Therapy

Ribonucleic acid (RNA) nanotechnology is a rapidly emerging field that focuses on the nanostructure design, construction, and application in biotechnology and biomedicine.

RNA Nanoparticle as a Safe and Effective Drug Delivery Platform for Cancer Therapy

Author: Sijin Guo

Publisher:

ISBN:

Page:

View: 636

Ribonucleic acid (RNA) nanotechnology is a rapidly emerging field that focuses on the nanostructure design, construction, and application in biotechnology and biomedicine. Unlike other biomacromolecules, RNA is more flexible in structure and more versatile in functionality. On the other hand, RNA is a naturally-occurring biopolymer, making them more biocompatible compared to other nanomaterials. Thus, RNA can serve as a building block to construct nanoparticles as drug delivery platform for cancer therapy. This dissertation primarily describes a fundamental study that explores the immune-compatibility of RNA nanoparticles, as well as the use of thermostable RNA nanoparticles to specifically deliver therapeutics for efficient cancer therapy without causing toxicity. Firstly, RNA polygons that have identical size but varying shapes, or same shape but with different sizes were constructed as study model. The RNA nanoparticles were found to be immunologically inert, indicating that RNA nanoparticles are safe drug carriers without triggering immune responses. On the other hand, they can elicit significant immunomodulation by extending the nanoparticles with special sequences. Specifically, this immunomodulation was found to be size, shape, sequence-dependent, demonstrating the potential of using RNA nanoparticles in immunotherapy. Secondly, the use of a thermostable RNA nanoparticle for solubilizing and high-density loading chemotherapeutic drugs for cancer inhibition is reported. Small chemotherapeutic drugs possess significant anti-cancer activity, but their clinical applications were greatly limited by the poor biocompatibility such as water-insolubility. By chemically conjugating water-insoluble drugs to RNA, the RNA nanoparticles dramatically improved drug water-solubility. An ultra-thermostable RNA four-way junction nanoparticle was able to covalently load twenty-four copies of paclitaxel without nanoparticle dissociation or unfolding. After intravenous administration, the resulting RNA-paclitaxel nanoparticles harboring specific cancer targeting ligand significantly inhibited breast cancer tumor in mice. The toxicity in organs and immune responses in mice were almost undetectable. Besides paclitaxel, this RNA-drug conjugation was applied to another anti-cancer drug, camptothecin, and showed significant inhibitory effect on tumor growth. Furthermore, an acid labile linker was developed within RNA-drug conjugates, aiming at controlling pH-responsive drug release in acidic tumor environment to improve drug efficacy. Thirdly, a three-dimensional RNA cube was constructed and explored as a multi-functional drug carrier by incorporation with different therapeutic modules. This multi-functional RNA cube is able to load chemotherapeutic drug, immunostimulatory molecule, or short-interfering RNA for enhanced cellular effects in vitro. What’s more, the RNA cube can be manipulated to become reconfigurable, suggesting the great potential of drug encapsulation and release for in vivo applications. Collectively, these findings demonstrate that RNA nanoparticles can serve as a biocompatible drug delivery platform for effective cancer therapy with favorable safety profile.

Journal of Nanoscience and Nanotechnology

The levels of fluorescence are wer than those seen for plasmid DNA delivery , a
result at is not entirely unexpected . It was found that pinocytic ading of the cells
with FDG frequently lead to osmotic pture of the cellular membrane . This fact ...

Journal of Nanoscience and Nanotechnology

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Nucleic Acids as Gene Anticancer Drug Delivery Therapy

This book presents the use of nanotechnology for medical applications, focusing on its use for anticancer drug delivery.

Nucleic Acids as Gene Anticancer Drug Delivery Therapy

Author: Loutfy H. Madkour

Publisher: Academic Press

ISBN: 0128197781

Page: 650

View: 822

Nucleic Acids as Gene Anticancer Drug Delivery Therapy highlights the most recent developments in cancer treatment using nucleic acids, nanoparticles and polymer nanoparticles for genomic nanocarriers as drug delivery, including promising opportunities for targeted and combination therapy. The development of a wide spectrum of nanoscale technologies is beginning to change the scientific landscape in terms of disease diagnosis, treatment, and prevention. This book presents the use of nanotechnology for medical applications, focusing on its use for anticancer drug delivery. Various intelligent drug delivery systems such as inorganic nanoparticles and polymer-based drug delivery are discussed. The use of smart drug delivery systems seems to be a promising approach for developing intelligent therapeutic systems for cancer immunotherapies and is discussed in detail along with nucleic acid-targeted drug delivery combination therapy for cancer. Nucleic Acids as Gene Anticancer Drug Delivery Therapy will be a useful reference for pharmaceutical scientists, pharmacologiests, and those involved in nanotechnology and cancer research. Discusses intelligent drug delivery systems such as inorganic nanoparticles and polymer-based drug delivery Contains a comprehensive comparison of various delivery systems, listing their advantages and limitations Presents combination therapy as a new hope for enhancing current gene-based treatment efficacy

Nanotechnology Based Approaches for Targeting and Delivery of Drugs and Genes

full advantage of lung delivery, a suitable delivery system with appropriate
physicochemical properties is necessary and SLNs is most fitted on this ... Apart
from that, various biological barriers also present problems in nucleic acid
delivery.

Nanotechnology Based Approaches for Targeting and Delivery of Drugs and Genes

Author: Vijay Mishra

Publisher: Academic Press

ISBN: 0128097183

Page: 552

View: 200

Nanotechnology-Based Approaches for Targeting and Delivery of Drugs and Genes provides an overview of the important aspects of nanomedicine in order to illustrate how to design and develop novel and effective drug delivery systems using nanotechnology. The book is organized into three sections, beginning with an introduction to nanomedicine and its associated issues. Section two discusses the latest technologies in nanomedicine, while the third section covers future developments and challenges in the field. By focusing on the design, synthesis, and application of a variety of nanocarriers in drug and gene delivery, this book provides pharmaceutical and materials science students, professors, clinical researchers, and industry scientists with a valuable resource aimed at tackling the challenges of delivering drugs and genes in a more targeted manner. Explores a wide range of promising approaches for the diagnosis and treatment of diseases using the latest advances in cutting-edge nanomedical technologies Contains contributions from world-renowned experts and researchers working in the area of nanomedicine and drug delivery Covers the associated challenges and potential solutions to working with nanotechnology in drug delivery Highlights crucial topics, such as biopharmaceutical and toxicity issues, quality by design, drug targeting, and more

Nucleic Acid Nanotechnology

bent helix motif, 104 blunt-end stacking effects, 95 tetrathymidine loops, 97
crossover motifs (see Crossover motifs) DNA nanotechnology DNA walker, 151
drug delivery, 153, 154 functional templating, 149 irregular nanoconstruction,
142–145 ...

Nucleic Acid Nanotechnology

Author: Jørgen Kjems

Publisher: Springer Science & Business Media

ISBN: 3642388159

Page: 339

View: 159

This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.

DNA and RNA Nanobiotechnologies in Medicine Diagnosis and Treatment of Diseases

This book will provide latest insights in the functional potentials of ribonucleic acids in medine and the use of Spiegelmer and Spiegelzyme systems. It will also deal with a new type of delivery systems for cellular targeting.

DNA and RNA Nanobiotechnologies in Medicine  Diagnosis and Treatment of Diseases

Author: Volker A. Erdmann

Publisher: Springer Science & Business Media

ISBN: 3642368530

Page: 460

View: 900

This book will provide latest insights in the functional potentials of ribonucleic acids in medine and the use of Spiegelmer and Spiegelzyme systems. It will also deal with a new type of delivery systems for cellular targeting.

Advances and Challenges in Nanomedicine

Understanding the advances in nanomedicine to date and the challenges that still need to be overcome, will allow future research to improve on existing platforms and to address the current translational and regulatory limitations.

Advances and Challenges in Nanomedicine

Author: Susan Hua

Publisher: Frontiers Media SA

ISBN: 2889457451

Page: 155

View: 354

Nanotechnology is a multidisciplinary field that is revolutionizing the way we detect and treat damage to the human body. Nanomedicine applies nanotechnology to highly specific medical interventions for the prevention, diagnosis, and treatment of diseases. They are increasingly being used to overcome biological barriers in the body to improve the way we deliver compounds to specific tissues and organs. In particular, nanomedicines have been shown to be beneficial for stabilizing therapeutic compounds, overcoming obstacles to cellular and tissue uptake, and improving biodistribution of compounds to target sites in vivo. Nanomedicines have demonstrated significant therapeutic advantages for a multitude of biomedical applications, however the clinical translation of these nanotechnology platforms has not progressed as quickly as the plethora of positive results would have suggested. Understanding the advances in nanomedicine to date and the challenges that still need to be overcome, will allow future research to improve on existing platforms and to address the current translational and regulatory limitations. This eBook “Advances and Challenges in Nanomedicine” has brought together experts in the fields of nanomedicine, nanotechnology, nanotoxicology, pharmaceutics, manufacturing, and translation to discuss the application of nanotechnology to drug delivery. This information is presented as original research, opinion, perspective, and review articles. The goal of this eBook is to generate collaborative discussion on the current status, general trends, challenges, strategies, and future direction of pharmaceutical nanotechnology, as well as highlight current and emerging nanoparticulate platforms with potential medical applications.

The Year in Neurology

GNA : DNA chemical cousin is a nanotechnology building block . Sci . Blog . Sci .
2 , http://www.scientificblogging.com/news'releases/ . Accessed on 2.20.2009 . 73
. Simpkins J.W. & N. Bodor . 1994. Brain - targeted delivery of dopamine using ...

The Year in Neurology

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Chemical Biology of Nucleic Acids

This volume contains 29 engrossing chapters contributed by worldwide, leading research groups in the field of chemical biology.

Chemical Biology of Nucleic Acids

Author: Volker A. Erdmann

Publisher: Springer Science & Business

ISBN: 3642544525

Page: 599

View: 602

This volume contains 29 engrossing chapters contributed by worldwide, leading research groups in the field of chemical biology. Topics include pre-biology; the establishment of the genetic code; isomerization of RNA; damage of nucleobases in RNA; the dynamic structure of nucleic acids and their analogs in DNA replication, extra- and intra-cellular transport; molecular crowding by the use of ionic liquids; new technologies enabling the modification of gene expression via editing of therapeutic genes; the use of riboswitches; the modification of mRNA cap regions; new approaches to detect appropriately modified RNAs with EPR spectroscopy and the use of parallel and high-throughput techniques for the analysis of the structure and new functions of nucleic acids. This volume discusses how chemistry can add new frontiers to the field of nucleic acids in molecular medicine, biotechnology and nanotechnology and is not only an invaluable source of information to chemists, biochemists and life scientists but will also stimulate future research.

Handbook of Pharmaceutical Biotechnology

DNA - based therapeutics and DNA delivery systems : A comprehensive review .
Aaps J. ... Controllable self - assembly of nanoparticles for specific delivery of
multiple therapeutic molecules to cancer cells using RNA nanotechnology . Nano
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Handbook of Pharmaceutical Biotechnology

Author: Shayne C. Gad

Publisher: Wiley-Interscience

ISBN:

Page: 1659

View: 641

Describes the use of biotechnology to develop pharmaceuticals. This book gives the professional a basic tool to facilitate the development of biotech medicines by bringing together a general overview of biotechnology used in the drug development process, along with a compendium of regulations and validation methods.

OECD Journal

RNA interference RNA interference ( also known as RNAi , small interference
RNA , or siRNA ) is a , “ gene - silencing process in which double - stranded ...
Nanotechnology holds a great deal of promise as a novel drug delivery
technology .

OECD Journal

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Nanocarriers Drug Delivery System

This is a highly informative and prudently organized book, providing scientific insight for readers with an interest in nanotechnology.

Nanocarriers  Drug Delivery System

Author: Nirmal Shah

Publisher: Springer Nature

ISBN: 9813344970

Page: 483

View: 687

A suitable drug delivery system is an essential element in achieving efficient therapeutic responses of drug molecules. With this desirability in mind, the book unites different techniques through which extremely small-sized particles can be utilized as a successful carrier for curing chronic as well as life-threatening diseased conditions. This is a highly informative and prudently organized book, providing scientific insight for readers with an interest in nanotechnology. Beginning with an overview of nanocarriers, the book impetuses on to explore other essential ways through which these carriers can be employed for drug delivery to varieties of administrative routes. This book discusses the functional and significant features of nanotechnology in terms of Lymphatic and other drug targeting deliveries. The book is presenting depth acquaintance for various vesicular and particulate nano-drug delivery carriers, utilized successfully in Pharmaceutical as well as in Cosmeceutical industries along with brief information on their related toxicities. In addition, the work also explores the potential applications of nanocarriers in biotechnology sciences for the prompt and safe delivery of nucleic acid, protein, and peptide-based drugs. An exclusive section in the book illuminates the prominence and competent applicability of nanotechnology in the treatment of oral cancer. The persistence of this book is to provide basic to advanced information for different novel carriers which are under scale-up consideration for the extensive commercialization. The book also includes recent discoveries and the latest patents of such nanocarriers. The cutting-edge evidence of these nanocarriers available in this book is beneficial to students, research scholars, and fellows for promoting their advanced research.

Theory Techniques and Applications of Nanotechnology in Gene Silencing

This book focuses on various aspects of nanotechnology based gene silencing. The initial chapters detail the techniques available for in vitro and in vivo characterization of nanoparticles.

Theory  Techniques and Applications of Nanotechnology in Gene Silencing

Author: Surendra Nimesh

Publisher: River Publishers

ISBN: 8792329837

Page: 193

View: 676

Gene silencing mediated through short interfering RNAs (siRNAs) is rapidly emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability in physiological conditions limits its therapeutic potential, hence there arises a need to develop a delivery system which can protect and efficiently transport siRNA to the target cells. Nanotechnology based non-viral vectors have been proposed as a potential alternate. Various polymeric nanoparticles have been shown as suitable delivery candidates with high cellular uptake efficiencies and advantage of reduced cytotoxicity. These delivery vectors form condensed complexes with siRNA which in turn provides protection to siRNA from enzymatic degradation and further leads to enhanced tissue and cellular targeting. Nanoparticles prepared from various polymers like polyethylenimine, chitosan have been largely exploited, as they bear several advantages such as, easy manipulatibility, high stability, low cost and high payload. Though numerous publications have appeared in the literature, there was still need to get more information under one roof, as book compilation which could provide a systematic account of all the aspects of nanotechnology mediated gene silencing right from the preparation of nanoparticles to in vitro and in vivo characterization followed by various applications in vitro and in vivo models. This book focuses on various aspects of nanotechnology based gene silencing. The initial chapters detail the techniques available for in vitro and in vivo characterization of nanoparticles. In the later chapters, exhaustive details about various polymeric systems employed for gene silencing has been accounted. The important topics discussed in the book include: Introduction to Nanotechnology Physicochemical Characterization of Nanoparticles In Vitro and In Vivo Characterization of Nanoparticles Concept and Barriers to Gene Silencing Nanoparticles Mediated Targeted siRNA delivery Polymers for siRNA Delivery Chitosan Polyethylenimine Poly-L-lysine Atelocollagen Protamine Dendrimers Cyclodextrin Poly (D, L-lactide-co-glycolide) Nanomaterials for siRNA delivery

OIE Bulletin

lorum Jorum OTE news Looking to the future : potential nanotechnology ... The
practicalities of delivery of RNA molecules to animals are likely to be common to
those addressed Both antisense and RNAi molecules evoke normal during ...

OIE Bulletin

Author: International Office of Epizootics

Publisher:

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