Engineering Tuneable Gene Circuits in Yeast

Imprint: Copyright © 2011 GRIN Verlag, Open Publishing GmbH ISBN: 978-3-668
-04548-4 This book at GRIN: http://www.grin.com/en/e-book/306639/engineering-
tuneable-gene-circuits-in-yeast Engineering Tuneable Gene Circuits in Yeast ...

Engineering Tuneable Gene Circuits in Yeast

Author: Stephen Checkley

Publisher: GRIN Verlag

ISBN: 3668045488

Page: 268

View: 277

Doctoral Thesis / Dissertation from the year 2011 in the subject Chemistry - Bio-chemistry, grade: pass, University of Manchester (Manchester Institute of Biotechnology), course: Systems biology doctoral training program -synthetic biology, language: English, abstract: Synthetic biology is an emergent field incorporating aspects of computer science, molecular biology-based methodologies in a systems biology context, taking naturally occurring cellular systems, pathways, and molecules, and selectively engineering them for the generation of novel or beneficial synthetic behaviour. This study described the construction of a novel synthetic gene circuit with interchangeable gene expression components enabling the investigation of tuning circuits for optimal signal to noise ratio. The circuit utilises the inducible downstream transcriptional activation properties of the pheromone-response pathway in the budding yeast Saccharomyces cerevisiae as the basis for initiation.

Fungal Biotechnology for Biofuel Production

... transcriptional outcome, providing an additional tunable component to be used
in synthetic circuits. Interestingly, those constructions also displayed a stochastic
gene expression behavior [119], which could be a drawback of this engineering ...

Fungal Biotechnology for Biofuel Production

Author: Roberto Nascimento Silva

Publisher: Bentham Science Publishers

ISBN: 1681080745

Page: 435

View: 240

Mycology: Current and Future Developments is a book series that brings together the latest contributions to research on the biology, genetics, and industrial use of fungi. Each book chapter is written by academic / professional experts from around the world. The book series is of interest to mycologists and allied researchers seeking to gain new knowledge perspectives about fungi. This volume of the book series focuses chiefly on advances biofuel production. Topics covered in this volume include an overview of biofuel production, the use of lignocelluloses in fungal biofuel production, fungal metabolic engineering, biomass pretreatment for biofuel refineries, and more. The volume also contains chapters about research on other fungi such as S. Cerevisiae. The reviews presented in this volume serve as a useful reference for researchers and readers interested in learning about new developments in biofuel production at a time when the need for alternative energy sources is ever increasing.

Advances in the Regulation and Production of Fungal Enzymes by Transcriptomics Proteomics and Recombinant Strains Design

Over the last years, the demand to expand the molecular toolbox for T. reesei to
facilitate genetic engineering and improve ... With the advances in genomics and
transcriptomics the identification of new constitutive and tunable promoters with
different expression strength was simplified. ... Synthetic expression systems
constitute a new option to control gene expression and build up complex gene
circuits.

Advances in the Regulation and Production of Fungal Enzymes by Transcriptomics  Proteomics and Recombinant Strains Design

Author: André Damasio

Publisher: Frontiers Media SA

ISBN: 2889630536

Page:

View: 652

Several efforts have been made in developing strategies to supply the enzyme market, as well as in reducing its costs. It includes the selection of an appropriate enzyme source and the optimization of enzyme properties and secretion. Carbohydrate-Active Enzymes (CAZymes) are industrially relevant biocatalysts that are capable of degrading plant cell wall biomass. The most important secreted enzymes related to plant cell wall decomposition are cellulases, hemicellulases, and auxiliary enzymes. These enzymes have been applied in the hydrolysis of plant biomass for the production of second-generation (2G) ethanol and several other high added value products. One of the bottlenecks for 2G ethanol production is the cost of enzymes applied on plant biomass hydrolysis. The improvement of proteins production by fungi applying system biology and genetic engineering is an interesting and promising strategy to reduce the enzymes cost and make the 2G ethanol production viable. Fungi play an important role in plant biomass degradation and biotechnology by producing and secreting high yields of enzymes. In spite of the fact that filamentous fungi present several advantages compared to other microorganisms due to the high level of proteins production, heterologous protein production is far from optimal levels and still needs to be improved. Currently, heterologous production of certain proteins is generally considerably lower than the levels obtained to homologous production. Many strategies have been studied in order to improve heterologous production of proteins by filamentous fungi, including the deletion of genes that encode for proteases, the deletion of lectin-like ER-Golgi cargo receptors and the co-expression of specific chaperones. It has been shown that the main bottleneck in the production of heterologous proteins is not caused by the low expression of the target gene. An experimental evidence suggests that most target proteins produced in filamentous fungi are lost or stuck in the secretory pathway due to errors in processing, modification or misfolding that result in their elimination by endoplasmic reticulum (ER) quality control. Misfolded proteins alter homeostasis and proper ER functioning resulting in a state known as ER stress. ER stress activates a conserved signaling pathway called unfolded protein response (UPR) and ER-associated protein degradation (ERAD), which upregulates genes responsible for restoring protein folding homeostasis in the cell and degrades misfolded protein in the cytosol by the ubiquitin-proteasome system. The genetic manipulation of individual genes and changes in the genome seems not to be the best alternative to overcome the main bottlenecks in heterologous protein secretion. However, the understanding of complex interactions of important proteins and genes, as well as how they are regulated is more promising.

Microbial Cell Factories Engineering for Production of Biomolecules

One such example is the time-reliant flocculation of yeast that simplifies the
separation of cells from the alcohol produced ... Upon subcutaneous implantation
in mice and consumption of the trigger molecules at a concentration, VEGF was
released in a dose-adjustable manner (K ... A robust and reliable method of
integrating individual genetic circuits into biological systems with predictable
behavior has ...

Microbial Cell Factories Engineering for Production of Biomolecules

Author: Vijai Singh

Publisher: Academic Press

ISBN: 0128214783

Page: 488

View: 622

Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others. This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields. Offers basic understanding and a clear picture of various MCFs Explains several tools and technologies, including DNA synthesis, synthetic biology tools, genome editing, biosensors, computer-aided design, and OMICS tools, among others Harnesses the potential of engineered MCFs to produce a wide range of biomolecules for industrial, therapeutic, pharmaceutical, nutraceutical and biotechnological applications Highlights the advances, challenges, and future opportunities in designing MCFs

Latest Advances in Glycoengineering

Another notable example from the Weiss group explored the use of CRISPR/
Cas9 to implement synthetic gene circuits in CHO cells, allowing tunable N-
glycan profiles of CHO culture-derived IgGs in a small molecule concentration-
dependent ...

Latest Advances in Glycoengineering

Author: Yanmei Li

Publisher: Frontiers Media SA

ISBN: 2889661202

Page:

View: 464

Glycans have long been known to be one of the most abundant biological molecules in living organisms. They can function as energy compounds, form structural cell wall/matrix polymers, or exist as oligomers that are attached on proteins, lipids and natural products to influence their properties and function. Because of their important biological roles, glycans have great potential for applications in the development of new drugs, materials, food additives and many other products. However, it is often difficult to directly obtain glycans from natural sources with ideal properties for these applications. Thus, modification of glycan structures for desired properties has emerged as an active area of research. This research area is generally called glycoengineering.

Synthetic Biology 2 Volume Set

3 5 6 7 8 9 Lu, T.K. (2010) Engineering scalable biological systems. Bioeng.
Bugs, 1 ... 11 Nevozhay, D., Zal, T., and Balazsi, G. (2013) Transferring a
synthetic gene circuit from yeast to mammalian cells. Nat. ... Nissim, L. and Bar-
Ziv, R.H. (2010) A tunable dual-promoter integrator for targeting of cancer cells.
Mol. Syst. Biol.

Synthetic Biology  2 Volume Set

Author: Robert A. Meyers

Publisher: John Wiley & Sons

ISBN: 352766856X

Page: 762

View: 927

These two volumes contain a selection of updated articles from the acclaimed Meyers Encyclopedia of Molecular Cell Biology and Molecular Medicine, the most authoritative resource in cell and molecular biology, combined with new articles by "founding fathers" in the field. The work is divided into six sections: + Biological Basis + Modeling + Modular Parts and Circuits + Synthetic Genomes + Diseases and Therapeutics + Chemicals Production. Ideally suited as advanced reading for students and postdocs, and with all current research trends covered by an impressive number of leading figures in the field, this is the first choice reference for research institutions.

NMR in Physiology and Biomedicine

Schena, M., Picard, D., and Yamamoto, K. R. (1991) Vectors for constitutive and
inducible gene expression in yeast. ... Vectors for expression of cloned genes in
yeast: Regulation, overproduction, and underproduction. ... in tissues with high
and fluctuating energy demands: The 'phosphocreatine circuit' for cellular energy
homeostasis. ... The genetic engineering of production traits in domestic animals.

NMR in Physiology and Biomedicine

Author: Robert J. Gillies

Publisher:

ISBN:

Page: 471

View: 275

Collected works of a noted Pakistani poet, particularly songs without musical notation.

Dissertation Abstracts International

The sciences and engineering. B. Dissertation ... 238B Tumor suppressor genes
Functional characterization of the Mnni tumour suppressor gene in Drosophila
melanogaster . ... 554B Tunable piezoelectric circuitry Vibration - based structural
damage identification enhancement via piezoelectric circuitry network and active
feedback control . ... 1 July 203 - B U3 snoRNA U3 snoRNA in a fission yeast .

Dissertation Abstracts International

Author:

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The Engineer

For instance, a thermometer may complete the circuit of an electro-magnet, the
armature of which inserts resistance into the leading circuit as the temperature
rises. ... A commwrvication from Geneste Hercher, and Co., Paris. ... The lever of
the governor is provided with an adjustable weight, by which means the speed of
the engine may be altered, or the ... Relates to a “dry” battery, the vexciting paste
of which is composed of plaster of Paris, chloride of sodium, sal-ammoniac, and
yeast.

The Engineer

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Nature

LETTERS 2010 A fast , robust and tunable synthetic gene oscillator ELEWI IN a +
Arabinose 12 Tt . arac 8 Fluorescence ( a.u. ) 4 180 0 . 2 Figure 1 Oscillations in
the dual - feedback circuit . a , Network diagram of the dual - feedback oscillator .
... and the differential activity of the two engineering - based approaches that
enable the construction of feedback loops can ... We placed the arac , lack and
yem GFP ( monomeric yeast - enhanced green fluorescent protein ) genes under
the ...

Nature

Author: Sir Norman Lockyer

Publisher:

ISBN:

Page:

View: 497

New Scientist

TUNABLE POLYMER COULD MAKE COLOURED LIGHT - EMITTING DISPLAYS
( 21 Mar 219 ) 879 .. THE SECRET LANGUAGE OF ... CUTTING OUT A HAZARD
OF GENETIC ENGINEERING ( 1 Feb p23 ) 136 .. ROBOTS AT THE READY ...

New Scientist

Author:

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American Artisan

A Weekly Journal of Arts, Mechanics, Manufactures, Engineering, Chemistry,
Inventions, and Patents. Ps . Ps . Hoxe ... ADJUSTABLE METALLIO DASH For
VEHICLE8 . - George M. ... Charles J. A. Dix , Eugene O'Brien , assignors to
Henry Pooley & Son , Liver- 4,865 . - PROVIDING ... YEAST FOR BREWERS
AND OTHERS . - Joseph Wolff ... 125,587 . - TELEGRAPHING BY CONSTANT
CIRCUITS .

American Artisan

Author:

Publisher:

ISBN:

Page:

View: 664