Capillary Electrophoresis of Proteins and Peptides

In Capillary Electrophoresis of Proteins and Peptides, topics range from recently developed approaches for both capillary coatings and analyte detection via laser, to the utilization of CE for the characterization of protein interactions ...

Capillary Electrophoresis of Proteins and Peptides

Author: Mark A. Strege

Publisher: Springer Science & Business Media

ISBN: 159259798X

Page: 332

View: 570

Capillary Electrophoresis (CE) has matured to become a superb complement to HLPC, evolving in some cases into an automated and quantitative replacement for conventional lab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. In Capillary Electrophoresis of Proteins and Peptides, topics range from recently developed approaches for both capillary coatings and analyte detection via laser, to the utilization of CE for the characterization of protein interactions with ligans, other proteins, and large biopolymers.

Capillary Electrophoresis of Proteins

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory.

Capillary Electrophoresis of Proteins

Author: Tim Wehr

Publisher: CRC Press

ISBN: 1482289857

Page: 300

View: 789

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

Capillary Electrophoresis of Proteins and Peptides

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins.

Capillary Electrophoresis of Proteins and Peptides

Author: Nguyet Thuy Tran

Publisher: Humana

ISBN: 9781493981533

Page: 234

View: 197

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.

Capillary Electrophoresis of Proteins and Peptides

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins.

Capillary Electrophoresis of Proteins and Peptides

Author: Nguyet Thuy Tran

Publisher: Humana Press

ISBN: 9781493940127

Page: 234

View: 280

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.

Capillary Electrophoresis of Proteins

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory.

Capillary Electrophoresis of Proteins

Author: Tim Wehr

Publisher: CRC Press

ISBN: 9780824702052

Page: 300

View: 966

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

Capillary Electrophoresis Mass Spectrometry

This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins.

Capillary Electrophoresis Mass Spectrometry

Author: James Q. Xia

Publisher: Springer

ISBN: 3319462407

Page: 74

View: 961

This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins. The application of capillary electrophoresis-mass spectrometry (CE-MS) coupling technology in the analysis of recombinant therapeutic proteins is detailed thoroughly. Specific topics include recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics, top-down analysis of monoclonal antibody using the CE-MS platform, and detection of host cell protein impurities. Comprehensive characterization of antibody-drug conjugates (ADCs) by coupling capillary electrophoresis with mass spectrometry is also covered. This is an ideal book for scientists in the life science and biopharmaceutical industry who are working on characterizing the PTMs of monoclonal antibodies, as well as graduate students and researchers in the separation science and biological mass spectrometry fields.

Capillary Electrophoresis

References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations. This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE).

Capillary Electrophoresis

Author: Paul D. Grossman

Publisher: Academic Press

ISBN: 0323138195

Page: 352

View: 718

This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations. This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on: Electroosmosis, separation efficiency, and Joule heating Detection methods In-depth discussion of the separation principles and capabilities of the major modes of CE Sieving gel electrophoresis Isoelectric focusing Free solution CE Micellar electrokinetic capillary chromatography Entangled polymer matrix-based separation Detailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations

Gel Electrophoresis of Proteins

This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors.

Gel Electrophoresis of Proteins

Author: B. D. Hames

Publisher: OUP Oxford

ISBN: 0191565636

Page: 372

View: 611

This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods were presented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.

Practical Capillary Electrophoresis

The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents.

Practical Capillary Electrophoresis

Author: Robert Weinberger

Publisher: Elsevier

ISBN: 0080539343

Page: 462

View: 177

In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Illustrated with detailed diagrams of electrophoretic phenomena Offers step-by-step methods development schemes Presents techniques for developing quantitative, robust, and precise methods Includes an extensive troubleshooting guide Updates and greatly expands on the first edition-more than 50% of the text is new Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE

Capillary Electrophoresis of Proteins

If this treatment is unsuccessful, the capillary tips can be immersed in a sonic
bath containing deionized water for several minutes. Surfactants can also be
added to improve protein solubility. Surfactants should be selected for their
solubilizing ...

Capillary Electrophoresis of Proteins

Author: Tim Wehr

Publisher: CRC Press

ISBN: 9780824702052

Page: 300

View: 127

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

The Application of Capillary Electrophoresis to Examine Protein Modifications in Baked Versus Fried Tortilla Chips

Although lipid oxidation is recognized as a major chemical reaction limiting shelf life of foods, its role in degrading food quality and the mechanisms involved remain incompletely elucidated.

The Application of Capillary Electrophoresis to Examine Protein Modifications in Baked Versus Fried Tortilla Chips

Author: Lisa Hayley Schultz

Publisher:

ISBN:

Page: 112

View: 791

Although lipid oxidation is recognized as a major chemical reaction limiting shelf life of foods, its role in degrading food quality and the mechanisms involved remain incompletely elucidated. Interactions of oxidizing lipids with other food molecules have been largely ignored, even though these reactions can have dramatic impact on food properties. Lipid co-oxidation of proteins occurs extensively in nearly all processed foods and degrades textures, flavors, color, and nutritional value. It is important to measure both lipid and protein co-oxidation products to understand the full extent of oxidative deterioration during food storage. This thesis is part of a larger project examining baked and fried tortilla chips to differentiate thermal damage to proteins from lipid co-oxidation during processing and storage. In a previous study, gel electrophoresis revealed modification of protein surfaces that affected dye binding, as well as formation of sizeable protein aggregates too large to enter normal gels involving disulfide, free radical, and other crosslinks. As an alternative to polyacrylamide gels, capillary electrophoresis can separate peptides without molecular weight limits, by modes that may be more sensitive to side chain modifications, and requires only a few nanoliters of sample. Thus, this study investigated the use of capillary electrophoresis for tracking fragmentation and crosslinking in co-oxidized proteins. Results corroborated observations that fried tortilla chip samples had greater changes than baked tortilla chip samples and higher incubation temperature resulted in more protein damage, most notably in fried reducing fractions. In addition, surface modifications altered protein charge, which interfered with migration in capillary electrophoresis. Peptide detection was limited to zeins of about 50 kDa because the sample filtration step intended to prevent capillary blockage also removed higher molecular weight fractions, including glutelins. However, fragmentation products not distinguishable in gel electrophoresis were detected. Overall, results of this study suggest that capillary electrophoresis has intriguing possibilities for supplementing SDS-PAGE and other protein analyses, particularly in verifying the presence of surface modifications. However, significant hurdles--such as reasons for lack of high molecular weight peptide loading and migration--remain to be overcome before capillary electrophoresis can become a primary method for analysis of modified proteins.