Androgen Action

The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, ...

Androgen Action

Author: Fahri Saatcioglu

Publisher: Humana Press

ISBN: 9781617792427

Page: 386

View: 668

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states.

Androgen Action

The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, ...

Androgen Action

Author: Fahri Saatcioglu

Publisher: Humana Press

ISBN: 9781493962013

Page: 389

View: 824

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states.

Androgen Action in Prostate Cancer

Finally, the androgen receptor is presented as a viable target for novel therapies. Together, these reviews should give the reader a comprehensive conceptual framework of androgen action in prostate cancer.

Androgen Action in Prostate Cancer

Author: Donald Tindall

Publisher: Springer Science & Business Media

ISBN: 9780387691794

Page: 826

View: 167

Androgens are critical regulators of prostate differentiation and function, as well as prostate cancer growth and survival. Therefore, androgen ablation is the preferred systemic treatment for disseminated prostate cancer. Androgen action is exerted in target tissues via binding the androgen receptor (AR), a nuclear receptor transcription factor. Historically, the gene expression program mediated by the AR has been poorly understood. However, recent gene expression profiling and more traditional single-gene characterization studies have revealed many androgen-regulated genes that are important mediators of androgen action in both normal and malignant prostate tissue. This book will focus on the androgen-regulated gene expression program, and examine how recently identified androgen-regulated genes are likely to contribute to the development and progression of prostate cancer. Recent studies that have attempted to unravel how these genes are deregulated in androgen depletion independent prostate cancer will be included

Androgen Action in Prostate Cancer

Finally, the androgen receptor is presented as a viable target for novel therapies. Together, these reviews should give the reader a comprehensive conceptual framework of androgen action in prostate cancer.

Androgen Action in Prostate Cancer

Author: Donald J. Tindall

Publisher: Springer

ISBN: 9780387691770

Page: 826

View: 340

Androgens are critical regulators of prostate differentiation and function, as well as prostate cancer growth and survival. Therefore, androgen ablation is the preferred systemic treatment for disseminated prostate cancer. Androgen action is exerted in target tissues via binding the androgen receptor (AR), a nuclear receptor transcription factor. Historically, the gene expression program mediated by the AR has been poorly understood. However, recent gene expression profiling and more traditional single-gene characterization studies have revealed many androgen-regulated genes that are important mediators of androgen action in both normal and malignant prostate tissue. This book will focus on the androgen-regulated gene expression program, and examine how recently identified androgen-regulated genes are likely to contribute to the development and progression of prostate cancer. Recent studies that have attempted to unravel how these genes are deregulated in androgen depletion independent prostate cancer will be included

STACS 93

Finally, the androgen receptor is presented as a viable target for novel therapies. Together, these reviews should give the reader a comprehensive conceptual framework of androgen action in prostate cancer.

STACS 93

Author: Patrice Enjalbert

Publisher: Springer Verlag

ISBN: 9780387565033

Page: 723

View: 480

"This volume contains the proceedings of the tenth annual Symposium on Theoretical Aspects of Computer Science (STACS '93), held in W}rzburg, February 25-27, 1993. The STACS symposia are held alternately in Germany and France, and organized jointly by the Special Interest Group for Theoretical Computer Science of the Gesellschaft f}r Informatik (GI) and theSpecial Interest Group for Applied Mathematics of the Association Francaise des Sciences et Technologies de l'Information et des Syst mes (afcet). The volume includes the three invited talks which opened the three days of the symposium: "Causal and distributed semantics for concurrent processes" (I. Castellani), "Parallel architectures: design and efficient use" (B. Monien et al.), and "Transparent proofs" (L. Babai). The selection of contributed papers is organized into parts on: computational complexity, logic in computer science, efficient algorithms, parallel and distributed computation, language theory, computational geometry, automata theory, semantics and logic of programming languages, automata theory and logic, circuit complexity, omega-automata, non-classical complexity, learning theory and cryptography, and systems."--PUBLISHER'S WEBSITE.

Mechanism of Androgen Action in the Prostate

Androgens are required for the structural and functional integrity of the prostate.

Mechanism of Androgen Action in the Prostate

Author: Feng Jiang

Publisher:

ISBN:

Page:

View: 686

Androgens are required for the structural and functional integrity of the prostate. Androgen action is intimately involved in the pathogenesis of two major prostate diseases, benign prostatic hyperplasia (BPH) and prostate carcinoma (CaP). Androgens regulate gene expression in the prostate through the androgen receptor (AR), a ligand-dependent transcription factor. To understand the molecular and cellular mechanisms of androgen action in the prostate, I used both cDNA subtractive hybridizations and microarray to comprehensively identify genes regulated by androgens using the rat ventral prostate model. Twenty-two genes up-regulated by androgens were isolated from my subtraction studies. From my microarray study, 162 and 143 genes were found to be up-regulated and down-regulated by androgens, respectively. Identification of these genes suggests that the following pathways are activated by androgens in the prostate---metabolism, protein synthesis, protein chaperoning and trafficking, secretions, cell cycle and apoptosis, and structural and extracellular proteins. I have characterized four androgen-responsive genes: FPPS, PLZF, GADD45gamma, and U19. FPPS is abundantly expressed and regulated by androgens in the rat prostatic epithelial cells. Both PLZF and GADD45gamma are found to be growth-suppressive to prostate cancer cells, suggesting that androgens might activate a signaling pathway to counteract the androgen-induced cell proliferation pathway. U19, a novel androgen-responsive apoptosis inducer, is also growth-suppressive to prostate cancer. Therefore, U19 was chosen for further mechanistic study. I identified FBI as a protein partner for U19. I determined that FB1 also interacts with EAF1, PML and ELL, and FB1 inhibits the transcriptional activity of U19. In conclusion, my thesis established a foundation for future mechanistic study of androgen action and provided new insights into the roles played by androgens in the prostate.

The Mechanisms of Androgen Action in Androgenetic Alopecia

Androgens gradually transform larger, terminal hair follicles in some scalp regions of genetically susceptible individuals into small, vellus ones, resulting in male pattern baldness.

The Mechanisms of Androgen Action in Androgenetic Alopecia

Author: Nigel Andrew Hibberts

Publisher:

ISBN:

Page: 277

View: 285

Androgens gradually transform larger, terminal hair follicles in some scalp regions of genetically susceptible individuals into small, vellus ones, resulting in male pattern baldness. Androgens are believed to act on the mainly epithelial hair follicle via the mesenchyme-derived dermal papilla. The aims of this study were to establish the culture of dermal papilla cells derived from balding scalp hair follicles and to investigate the mechanisms of androgen action in dermal papilla cells derived from balding and non-balding scalp in an attempt to understand how androgens inhibit terminal hair follicles. Dermal papillae from balding scalp follicles were considerably smaller and hence more difficult to culture than those from non-balding scalp. Primary dermal papilla cell lines derived from balding scalp showed several differences in vitro compared with those from non-balding scalp. Saturation analysis revealed the presence of high affinity, low capacity androgen receptors in all cell twpes, with balding scalp derrnal papilla cells having higher levels of androgen receptors than those from non-balding scalp. Competition studies confirmed the androgen specificity of these receptors. A putative, novel antiandrogen was unable to compete for the androgen receptor. Although androgens were unable to directly influence the growth of dermal papilla cells in vitro, in balding but not control non-balding cells, androgens were able to induce the production of factors which inhibited the growth of the continuous epithelial cell line, NCTC 2544. Cultured dermal papilla cells clearly exhibit an altered gene expression which is reflective of their in vivo origins and therefore appear to offer an excellent model system to study the effects of androgens on hair growth.

Androgen Responsive Genes in Prostate Cancer

This book is intended for individuals interested in cancer endocrinology and medical and healthcare professionals involved in prostate cancer research.

Androgen Responsive Genes in Prostate Cancer

Author: Zhou Wang

Publisher: Springer Science & Business Media

ISBN: 1461461820

Page: 348

View: 638

Androgens and androgen receptors (AR) play critical roles in the development and progression of prostate cancer, the most frequently diagnosed cancer and second leading cause of cancer death in US males. AR is an androgen-dependent DNA-binding transcription factor that regulates the expression of androgen-responsive genes. Identification and characterization of androgen-responsive genes provide insights into the cellular mechanisms of androgen action and may lead to new approaches in diagnosis, prognosis, prevention and/or treatment of prostate cancer. This volume provides critical information from well respected experts in the field. Some of the exciting topics include the new understanding of mechanisms underlining the regulation of androgen-responsive gene expression, and functions of various androgen-responsive genes in biological processes essential in carcinogenesis including cell growth, angiogenesis, and epithelial-to-mesenchyme transition (EMT). Other important aspects addressed are the current and potential clinic applications of knowledge on androgen-responsive gene regulation and function. This book is intended for researchers, scientists, faculty, and advanced graduate students with an interest in androgen action and prostate cancer.​

Androgens and Androgen Receptor

This book is of interest to students, basic scientists, and clinicians as both a study guide and reference of research in the androgen field. It could also be used as an advanced level text in endocrinology, urology, OBGYN, or oncology.

Androgens and Androgen Receptor

Author: Chawnshang Chang

Publisher: Springer Science & Business Media

ISBN: 1461511615

Page: 503

View: 831

Androgen Receptors is the most comprehensive and up to date volume on the topic, including discussions of the basic mechanisms of androgen-androgen receptor actions, their roles in the androgen-related diseases, and their potential clinical applications. Key topics covered include: -The discovery and cloning of the androgen receptor; -Androgen receptor coregulators; -Androgen related genes and their consensus DNA response elements; -Basic mechanism of action including functional analyses, cellular localization and phosphorylation studies; -Cross-talk to other signal transduction systems; -The recent connections of androgens to women's diseases, such as osteoporosis and ovarian cancer. This book is of interest to students, basic scientists, and clinicians as both a study guide and reference of research in the androgen field. It could also be used as an advanced level text in endocrinology, urology, OBGYN, or oncology.

Testosterone

Testosterone provides the most comprehensive source of information on testosterone and its role in physiology and pathology.

Testosterone

Author: Eberhard Nieschlag

Publisher: Cambridge University Press

ISBN: 1107012902

Page: 582

View: 669

Testosterone provides the most comprehensive source of information on testosterone and its role in physiology and pathology.

Genetic Steroid Disorders

After reviewing the mechanisms of androgen action, we will look at the main clinical features of androgen insensitivity, as well as the biological, cellular, and molecular tools used to investigate the AR. Numerous AR mutations have been ...

Genetic Steroid Disorders

Author: Charles Sultan

Publisher: Elsevier Inc. Chapters

ISBN: 0128072911

Page: 406

View: 936

After reviewing the mechanisms of androgen action, we will look at the main clinical features of androgen insensitivity, as well as the biological, cellular, and molecular tools used to investigate the AR. Numerous AR mutations have been described over the past 20 years. We will consider the challenges for diagnosis and prognosis by examining the genotype–phenotype relationship and the possibility of somatic mutations. Last, we will address the role of genetic counseling and the elements that should be taken into account for the difficult decision of sex assignment in children affected with AIS.